منابع مشابه
Comet assay in myelodysplastic syndromes
The comet assay (single-cell gel electrophoresis) has been established as a simple, rapid, flexible and sensitive method of detecting DNA damage in single cells(1,2). Cells embedded in agarose on a microscope slide are lysed with detergent. Electrophoresis at high pH results in structures resembling comets, observed by fluorescence microscopy; the intensity of the comet tail relative to the hea...
متن کاملRecommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop.
The in vivo alkaline single cell gel electrophoresis assay, hereafter the Comet assay, can be used to investigate the genotoxicity of industrial chemicals, biocides, agrochemicals and pharmaceuticals. The major advantages of this assay include the relative ease of application to any tissue of interest, the detection of multiple classes of DNA damage and the generation of data at the level of th...
متن کاملStatistical analysis of comet assay results
The comet assay can distinguish small differences in DNA damage between different samples of cells, implying that statistical tests are important to assess whether this occurs by chance. Excellent scholarly papers with concise descriptions of statistical analysis and recommendations for tests have been published (Lovell et al., 1999; Lovell and Omori, 2008). We often come across publications th...
متن کاملThe comet assay: a heavenly method!
The contributions to this special issue of Mutagenesis have been selected to cover the main research areas served by the comet assay, namely genotoxicology, environmental toxicology, human biomonitoring and fundamental investigations into mechanisms of DNA damage and repair. Innovative methods are described, technical issues are explored, and guidelines are given for venturing into relatively n...
متن کاملDNA Repair Measured by the Comet Assay
The stability of the genome is of crucial importance, and yet the DNA molecule is prone to spontaneous loss of bases, and damage from exogenous and endogenous sources – with potentially mutagenic consequences. Damage can take the form of small alterations to bases (alkylation or oxidation); breaks in the sugar-phosphate backbone involving one or both strands (single or double strand breaks – SS...
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ژورنال
عنوان ژورنال: MethodsX
سال: 2020
ISSN: 2215-0161
DOI: 10.1016/j.mex.2020.101161